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KMID : 0880220150530010014
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Journal of Microbiology
2015 Volume.53 No. 1 p.14 ~ p.20
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Genetic diversity of Clavispora lusitaniae isolated from Agave fourcroydes Lem, as revealed by DNA fingerprinting
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Perez-Brito Daisy
Magana-Alvarez Anuar
Lappe-Oliveras Patricia
Cortes-Velazquez Alberto
Torres-Calzada Claudia
Herrera-Suarez Teofilo
Larque-Saavedra Alfonso
Tapia-Tussell Raul
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Abstract
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This study characterized Clavispora lusitaniae strains isolated from different stages of the processing and early fermentation of a henequen (Agave fourcroydes) spirit produced in Yucatan, Mexico using a molecular technique. Sixteen strains identified based on morphological features, obtained from different substrates, were typed molecularly. Nine different versions of the divergent D1/D2 domain of the large-subunit ribosomal DNA sequence were identified among the C. lusitaniae strains. The greatest degree of polymorphism was found in the 90-bp structural motif of the D2 domain. The MSP-PCR technique was able to differentiate 100% of the isolates. This study provides significant insight into the genetic diversity of the mycobiota present during the henequen fermentation process, especially that of C. lusitaniae, for which only a few studies in plants have been published. The applied MSP-PCR markers were very efficient in revealing olymorphisms between isolates of this species.
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KEYWORD
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Agave fourcroydes, Clavispora lusitaniae, D1/D2 large-subunit rDNA polymorphism, DNA fingerprinting, genetic diversity
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